The European food safety ecosystem is a community of diverse actors – part of a wider, global ecosystem – which creates and captures new value through both collaboration and competition. At the same time, the ecosystem evolves by way of knowledge sharing and innovation. Therefore, investments made in partnerships and cooperation in the said ecosystem are set to result in further economies of scale and more capacity to deliver on even the most complex regulatory science. The increase in scientific complexity, changing societal expectations, new policy and strategy initiatives, and new market trends create opportunities but also pose new challenges to the food safety ecosystem. These challenges trigger the need to rethink how the actors within the ecosystem can continue to ensure safe and healthy food and feed, and thereby also contribute to sustainable food systems and One Health goals. The Transparency Regulation has set up a new legal framework enhancing openness and transparency throughout the risk assessment process. It also provides the means for more comprehensive and timely scientific advice through collaboration and cooperation. This session will explore how each actor can help to address the outlined challenges to reach the overarching food and feed safety goals of the ecosystem, taking into account individual values, needs and possible limitations, and how the ecosystem approach can directly impact on the quality of regulatory science. We will also explore new ways of engagement, cooperation, and collaboration among the ecosystem actors, and how they can contribute to more fit-for-purpose and trusted food and feed safety advice.