In support of the EU Green Deal, the so-called ENVI Agencies (ECDC, ECHA, EEA, EFSA and EMA) contribute to a sustainable One Health approach. In the coming decade, billions of euros will be dedicated to Research & Innovation (R&I) needed to support a transition towards more sustainability. EU Agencies, individually and collectively, can make a difference in supporting the EU Research Agenda. During the thematic session, we will look at their peculiar role at the crossroads of the science–policy interface, and will discuss their current and envisioned research involvement. We will present case studies showing the benefits of EU Agency involvement in research and will formulate recommendations on how they could be involved even more and thus generate additional value. EU Agencies that work closely together, in research and other areas, provide the EU with more holistic answers, which are needed for our society and the environment.